[cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri)].
نویسندگان
چکیده
In this paper, partial sequences of the tree shrew (Tupaia belangeri) Klf4, Sox2, and c-Myc genes were cloned and sequenced, which were 382, 612, and 485 bp in length and encoded 127, 204, and 161 amino acids, respectively. Whereas, their cDNA sequence identities with those of human were 89%, 98%, and 89%, respectively. Their phylogenetic tree results indicated different topologies and suggested individual evolutional pathways. These results can facilitate further functional studies.
منابع مشابه
[Molecular cloning of Tupaia belangeri chinensis neuropeptide Y and homology comparison with other analogues from primates].
Much attention has been payed to tree shrews for their close phylogenetic relationship with primates, small size, and short reproductive cycle. Especially, they are considered as excellent experiential animals for medicine or/and disease research. A nucleotide sequence encoding neuropeptide Y(NPY) precursor has been cloned from the cDNA library of Tupaia belangeri chinensis. Sequence alignment ...
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The tree shrews (Tupaia belangeri) diverged from the primate order (Primates) and are classified as Scandentia, a separate taxonomic group of mammals. The tree shrew has been suggested to use an animal model to study human disease but the genomic sequences of tree shrew is largely unidentified. Here we identified the full-length cDNA sequence of a housekeeping gene, Glyceraldehyde 3-phosphate D...
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متن کاملIdentification of the full-length β-actin sequence and expression profiles in the tree shrew (Tupaia belangeri).
The tree shrew (Tupaia belangeri) diverges from the primate order (Primates) and is classified as a separate taxonomic group of mammals - Scandentia. It has been suggested that the tree shrew can be used as an animal model for studying human diseases; however, the genomic sequence of the tree shrew is largely unidentified. In the present study, we reported the full-length cDNA sequence of the h...
متن کاملExtensive characterization of Tupaia belangeri neuropeptidome using an integrated mass spectrometric approach.
Neuropeptidomics is used to characterize endogenous peptides in the brain of tree shrews (Tupaia belangeri). Tree shrews are small animals similar to rodents in size but close relatives of primates, and are excellent models for brain research. Currently, tree shrews have no complete proteome information available on which direct database search can be allowed for neuropeptide identification. To...
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ورودعنوان ژورنال:
- Dong wu xue yan jiu = Zoological research
دوره 34 2 شماره
صفحات -
تاریخ انتشار 2013